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There is a discrepancy in the reported concentration between nta and evquant

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There is a discrepancy between measurements based on nanoparticle tracking analysis and the EVQuant protocol, in which it is possible to quantifying the concentration of EVs in urine or plasma through confocal microscopy [@hartjes2020EVQuant; high-throughput quantification and characterization of extracellular vesicle (sub)populations].

This may be due to small particles are properly characterized by NanoSight (at least when small means liposomes of 40nm). While in a fluorescent confocal microscope, with an immobilized sample, it is possible to adjust the settings to become sensitive event o single-molecules.


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